| 质粒名称: | LZRS ERTm RasV12 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21199 |
| 存储实验室: | Paul Khavari |
|
载体骨架基本信息 |
|
| 载体名称: | LZRS no LacZ |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 11100bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EsR1- HRas |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 1700bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | LZRS ERTm RasV12 was a gift from Paul Khavari (Addgene plasmid # 21199 ; http://n2t.net/addgene:21199 ; RRID:Addgene_21199) |
| 参考文献部分: | Epidermal Ras blockade demonstrates spatially localized Ras promotion of proliferation and inhibition of differentiation. Dajee M, Tarutani M, Deng H, Cai T, Khavari PA. Oncogene. 2002 Feb 28;21(10):1527-38 10.1038/sj.onc.1205287 PubMed 11896581 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS LZRS ERTm RasV12 12382 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS LZRS ERTm RasV12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12382)
AUTHORS Dajee M, Tarutani M, Deng H, Cai T, Khavari PA
TITLE Epidermal Ras blockade demonstrates spatially localized Ras
promotion of proliferation and inhibition of differentiation
JOURNAL Oncogene. 2002 Feb 28;21(10):1527-38
PUBMED 11896581
REFERENCE 2 (bases 1 to 12382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12382
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1045..1614
/codon_start=1
/product="human oncoprotein generated by the G12V mutation
in the small GTPase H-Ras"
/label=H-Ras (G12V)
/translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
RQHKLRKLNPPDESGPGCMSCKCVLS"
LTR 1835..2304
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(2434..2451)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2605..3193)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2685..2704)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 3316..3450
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3340..3359)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3394..3413
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(3680..4279)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3763..3783)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 4260..4279
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(4362..4861)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4410..4429)
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
primer_bind 4803..4826
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
rep_origin 6893..8682
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(9176..10036)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9799..9818
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10037..10141)
/gene="bla"
/label=AmpR promoter
LTR 10678..11147
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 11212..11569
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 11634..12050
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 11980..12002
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 12018..12034
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 12060..52
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 12372..6
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
ORIGIN
1 cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatcc
61 accatgcgaa atgaaatggg tgcttcagga gacatgaggg ctgccaacct ttggccaagc
121 cctcttgtga ttaagcacac taagaagaat agccctgcct tgtccttgac agctgaccag
181 atggtcagtg ccttgttgga tgctgaaccg cccatgatct attctgaata tgatccttct
241 agacccttca gtgaagcctc aatgatgggc ttattgacca acctagcaga tagggagctg
301 gttcatatga tcaactgggc aaagagagtg ccaggctttg gggacttgaa tctccatgat
361 caggtccacc ttctcgagtg tgcctggctg gagattctga tgattggtct cgtctggcgc
421 tccatggaac acccggggaa gctcctgttt gctcctaact tgctcctgga caggaatcaa
481 ggtaaatgtg tggaaggcat ggtggagatc tttgacatgt tgcttgctac gtcaagtcgg
541 ttccgcatga tgaacctgca gggtgaagag tttgtgtgcc tcaaatccat cattttgctt
601 aattccggag tgtacacgtt tctgtccagc accttgaagt ctctggaaga gaaggaccac
661 atccaccgtg tcctggacaa gatcacagac actttgatcc acctgatggc caaagctggc
721 ctgactctgc agcagcagca tcgccgccta gctcagctcc ttctcattct ttcccatatc
781 cggcatatga gtaacaaacg catggagcat ctctacaaca tgaaatgcaa gaacgtggta
841 cccctctatg acctgctcct ggagatgttg gatgcccacc gccttcatgc cccagccagt
901 cgcatgggag tgcccccaga ggagcccagc cagacccagc tggccaccac cagctccact
961 tcagcacatt ccttacaaac ctactacata cccccggaag cagagggctt ccccaacgat
1021 atcccacgtg gaggcctgaa ttccatgacg gaatataagc tggtggtggt gggcgccgtc
1081 ggtgtgggca agagtgcgct gaccatccag ctgatccaga accattttgt ggacgaatac
1141 gaccccacta tagaggattc ctaccggaag caggtggtca ttgatgggga gacgtgcctg
1201 ttggacatcc tggataccgc cggccaggag gagtacagcg ccatgcggga ccagtacatg
1261 cgcaccgggg agggcttcct gtgtgtgttt gccatcaaca acaccaagtc ttttgaggac
1321 atccaccagt acagggagca gatcaaacgg gtgaaggact cggatgacgt gcccatggtg
1381 ctggtgggga acaagtgtga cctggctgca cgcactgtgg aatctcggca ggctcaggac
1441 ctcgcccgaa gctacggcat cccctacatc gagacctcgg ccaagacccg gcagggagtg
1501 gaggatgcct tctacacgtt ggtgcgtgag atccggcagc acaagctgcg gaagctgaac
1561 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgacgcaat
1621 tcgatatcaa gcttgaattc ccgcgggcgg ccgccagcac agtggtcgac gataaaataa
1681 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
1741 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
1801 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
1861 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
1921 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
1981 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
2041 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
2101 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
2161 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
2221 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
2281 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
2341 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
3301 tttgcggccg aattagatcc agacatgata agatacattg atgagtttgg acaaaccaca
3361 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
3421 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
3481 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
3541 atggctgatt atgatcctct agagtcggtg ggcctcgggg gcgggtgcgg ggtcggcggg
3601 gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc gctcctttcg gtcgggcgct
3661 gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
3721 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
3781 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
3841 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg
3901 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
3961 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
4021 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg
4081 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
4141 tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga
4201 acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg
4261 tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag gggtcagggg cgtgggtcag
4321 gggatggtgg cggcaccggt cgtggcggcc gacctgcagg tcgaaaggcc cggagatgag
4381 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
4441 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
4501 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
4561 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
4621 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
4681 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
4741 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
4801 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
4861 cggtagaatt aattctcatg tttgacagct tatcatcgat aagctgatcc tcacaggccg
4921 cacccagctt ttcttccgtt gccccagtag catctctgtc tggtgacctt gaagaggaag
4981 aggaggggtc ccgagaatcc ccatccctac cgtccagcaa aaagggggac gaggaatttg
5041 aggcctggct tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc
5101 tgcgagtaat tggtgatgag gacgaggatg gttcggagga tggggaattt tcagacctgg
5161 atctgtctga cagcgaccat gaaggggatg agggtggggg ggctgttgga gggggcagga
5221 gtctgcactc cctgtattca ctgagcgtcg tctaataaag atgtctattg atctctttta
5281 gtgtgaatca tgtctgacga ggggccaggt acaggacctg gaaatggcct aggagagaag
5341 ggagacacat ctggaccaga aggctccggc ggcagtggac ctcaaagaag agggggtgat
5401 aaccatggac gaggacgggg aagaggacga ggacgaggag gcggaagacc aggagccccg
5461 ggcggctcag gatcagggcc aagacataga gatggtgtcc ggagacccca aaaacgtcca
5521 agttgcattg gctgcaaagg gacccacggt ggaacaggag caggagcagg agcgggaggg
5581 gcaggagcag gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga
5641 ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg
5701 ggtcgaggag gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt
5761 cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt
5821 agtcagtcat catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt
5881 ttccaccctg taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt
5941 gagcctgacg tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc
6001 ccaagcactg gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt
6061 ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta
6121 agagctctcc tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc
6181 ggtgtgttcg tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc
6241 cttgctattc cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct
6301 ggacccggcc cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt
6361 ttacaaactc atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca
6421 aagcccgctc ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat
6481 ttgcctccct ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat
6541 gacggagatg aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg
6601 ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc
6661 cccagtagac atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt
6721 cgtcctccca acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc
6781 tcaacacctt ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg
6841 cgacggcttt agcctggcct ccttaaattc acctaagaat gggagcaacc agcaggaaaa
6901 ggacaagcag cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa aggatagcac
6961 tcccactcta ctactgggta tcatatgctg actgtatatg catgaggata gcatatgcta
7021 cccggataca gattaggata gcatatacta cccagatata gattaggata gcatatgcta
7081 cccagatata gattaggata gcctatgcta cccagatata aattaggata gcatatacta
7141 cccagatata gattaggata gcatatgcta cccagatata gattaggata gcctatgcta
7201 cccagatata gattaggata gcatatgcta cccagatata gattaggata gcatatgcta
7261 tccagatatt tgggtagtat atgctaccca gatataaatt aggatagcat atactaccct
7321 aatctctatt aggatagcat atgctacccg gatacagatt aggatagcat atactaccca
7381 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca
7441 gatataaatt aggatagcat atactaccca gatatagatt aggatagcat atgctaccca
7501 gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat atgctatcca
7561 gatatttggg tagtatatgc tacccatggc aacattagcc caccgtgctc tcagcgacct
7621 cgtgaatatg aggaccaaca accctgtgct tggcgctcag gcgcaagtgt gtgtaatttg
7681 tcctccagat cgcagcaatc gcgcccctat cttggcccgc ccacctactt atgcaggtat
7741 tccccggggt gccattagtg gttttgtggg caagtggttt gaccgcagtg gttagcgggg
7801 ttacaatcag ccaagttatt acacccttat tttacagtcc aaaaccgcag ggcggcgtgt
7861 gggggctgac gcgtgccccc actccacaat ttcaaaaaaa agagtggcca cttgtctttg
7921 tttatgggcc ccattggcgt ggagccccgt ttaattttcg ggggtgttag agacaaccag
7981 tggagtccgc tgctgtcggc gtccactctc tttccccttg ttacaaatag agtgtaacaa
8041 catggttcac ctgtcttggt ccctgcctgg gacacatctt aataacccca gtatcatatt
8101 gcactaggat tatgtgttgc ccatagccat aaattcgtgt gagatggaca tccagtcttt
8161 acggcttgtc cccaccccat ggatttctat tgttaaagat attcagaatg tttcattcct
8221 acactagtat ttattgccca aggggtttgt gagggttata ttggtgtcat agcacaatgc
8281 caccactgaa ccccccgtcc aaattttatt ctgggggcgt cacctgaaac cttgttttcg
8341 agcacctcac atacacctta ctgttcacaa ctcagcagtt attctattag ctaaacgaag
8401 gagaatgaag aagcaggcga agattcagga gagttcactg cccgctcctt gatcttcagc
8461 cactgccctt gtgactaaaa tggttcacta ccctcgtgga atcctgaccc catgtaaata
8521 aaaccgtgac agctcatggg gtgggagata tcgctgttcc ttaggaccct tttactaacc
8581 ctaattcgat agcatatgct tcccgttggg taacatatgc tattgaatta gggttagtct
8641 ggatagtata tactactacc cgggaagcat atgctacccg tttagggtta acaagggggc
8701 cttataaaca ctattgctaa tgccctcttg agggtccgct tatcggtagc tacacaggcc
8761 cctctgattg acgttggtgt agcctcccgt agtcttcctg ggcccctggg aggtacatgt
8821 cccccagcat tggtgtaaga gcttcagcca agagttacac ataaaggcaa tgttgtgttg
8881 cagtccacag actgcaaagt ctgctccagg atgaaagcca ctcagtgttg gcaaatgtgc
8941 acatccattt ataaggatgt caactacagt cagagaaccc ctttgtgttt ggtccccccc
9001 cgtgtcacat gtggaacagg gcccagttgg caagttgtac caaccaactg aagggattac
9061 atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc aggtttagtt cgtccggggc
9121 ggggatctgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
9181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
9241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
9301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
9361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
9421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
9481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
9541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
9601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
9661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
9721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
9781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
9841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
9901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
9961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
10021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
10081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
10141 gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg agtttcctaa tacatcccaa
10201 actcaaatat ataaagcatt tgacttgttc tatgccctag ggggcggggg gaagctaagc
10261 cagctttttt taacatttaa aatgttaatt ccattttaaa tgcacagatg tttttatttc
10321 ataagggttt caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata
10381 aaaatagata aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt
10441 gacaacataa atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta
10501 tgccagtcat attaattact agtcaattag ttgattttta tttttgacat atacatgtga
10561 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
10621 aaaatacata actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg
10681 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
10741 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
10801 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
10861 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
10921 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
10981 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
11041 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
11101 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
11161 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
11221 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
11281 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
11341 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
11401 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
11461 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
11521 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
11581 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
11641 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
11701 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
11761 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
11821 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
11881 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
11941 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
12001 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
12061 catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
12121 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
12181 acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
12241 ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
12301 aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
12361 acggcatcgc agcttggata ca
//
